Cyth2^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyth2^{tm1d(EUCOMM)Wtsi}
• Name: cytohesin 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7266327
• Synonyms: ARNO KO
• Gene: Cyth2 Location: Chr7:45456058-45463857 bp, - strand Genetic Position: Chr7, 29.52 cM, cytoband B3
• Alliance: Cyth2^{tm1d(EUCOMM)Wtsi} page
• IMPC: Cyth2 gene page

Mutation origin

• Mutant Cell Line: EPD0140_3_D11
• Germline Transmission: Earliest citation of germline transmission: J:307633
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 45459751 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 45460840. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:307633)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyth2 Mutation: 20 strains or lines available

References

• Original: J:307633 London NR Jr, et al., Deletion of Arno Reduces Eosinophilic Inflammation and Interleukin-5 Expression in a Murine Model of Rhinitis. Ear Nose Throat J. 2021 Jan 4;:145561320986055
• All: 1 reference(s)