Agmo^{tm2a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Agmo^{tm2a(EUCOMM)Wtsi}
• Name: alkylglycerol monooxygenase; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:7256325
• Synonyms: Agmo-lacZ tg
• Gene: Agmo Location: Chr12:37291640-37632201 bp, + strand Genetic Position: Chr12, 16.84 cM
• Alliance: Agmo^{tm2a(EUCOMM)Wtsi} page
• IMPC: Agmo gene page

Mutation origin

• Mutant Cell Line: EPD0354_2_F05
• Germline Transmission: Earliest citation of germline transmission: J:308367
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutations: Duplication, Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 37293227 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 37294023. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml The targeting event produced an unintended duplication of a 94 kb genomic region encompassing exons 1 through 3. (J:308367)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agmo Mutation: 24 strains or lines available

References

• Original: J:308367 Sailer S, et al., When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping. Cell Biosci. 2021 Mar 16;11(1):54
• All: 2 reference(s)