Ism1^em1.1Kajs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ism1^em1.1Kajs
• Name: isthmin 1, angiogenesis inhibitor; endonuclease-mediated mutation 1.1, Katrin J Svensson
• MGI ID: MGI:6827291
• Synonyms: Ism1-KO
• Gene: Ism1 Location: Chr2:139520098-139600501 bp, + strand Genetic Position: Chr2, 69.06 cM
• Alliance: Ism1^em1.1Kajs page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Introns 1 and 4 were targeted with sgRNAs and ssODN templates to insert loxP sites using CRISPR/Cas9 technology, resulting in the deletion of exons 2-4 after subsequent cre-mediated recombination. The loss of mRNA expression from this allele was confirmed by RT-qPCR. (J:309734)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ism1 Mutation: 26 strains or lines available

References

• Original: J:309734 Jiang Z, et al., Isthmin-1 is an adipokine that promotes glucose uptake and improves glucose tolerance and hepatic steatosis. Cell Metab. 2021 Sep 7;33(9):1836-1852.e11
• All: 1 reference(s)