Slco2b1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slco2b1^{tm1d(KOMP)Wtsi}
• Name: solute carrier organic anion transporter family, member 2b1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6489390
• Synonyms: OATP2B1^-, Oatp2b1-deficient
• Gene: Slco2b1 Location: Chr7:99307011-99360547 bp, - strand Genetic Position: Chr7, 54.11 cM
• Alliance: Slco2b1^{tm1d(KOMP)Wtsi} page
• IMPC: Slco2b1 gene page

Mutation origin

• Mutant Cell Line: EPD0099_1_B03
• Germline Transmission: Earliest citation of germline transmission: J:287311
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 99337797 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 99338675. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287311)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slco2b1 Mutation: 34 strains or lines available

References

• Original: J:287311 Chen M, et al., Role of Oatp2b1 in Drug Absorption and Drug-Drug Interactions. Drug Metab Dispos. 2020 May;48(5):420-426
• All: 2 reference(s)