Wfdc2^em1Fcw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Wfdc2^em1Fcw
• Name: WAP four-disulfide core domain 2; endonuclease-mediated mutation 1, Fengchao Wang
• MGI ID: MGI:6476955
• Synonyms: wfdc2^-
• Gene: Wfdc2 Location: Chr2:164404635-164410430 bp, + strand Genetic Position: Chr2, 85.2 cM, cytoband H3
• Alliance: Wfdc2^em1Fcw page

Mutation origin

• Strain of Origin: C57

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 1 was targeted with two TALENs (GCACCATGCCTGCCTG and AGTAGGAGGCCAGCGGC), resulting in a 17 bp deletion (TCGCCTCTGCTTGCTGG). qRT-PCR and Western blots showed absence of mRNA and protein expression from this allele. (J:293623)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wfdc2 Mutation: 17 strains or lines available

References

• Original: J:293623 Zhang T, et al., WFDC2 gene deletion in mouse led to severe dyspnea and type-I alveolar cell apoptosis. Biochem Biophys Res Commun. 2020 Feb 5;522(2):456-462
• All: 1 reference(s)