Dpp3^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Dpp3^{tm1d(EUCOMM)Hmgu}
• Name: dipeptidylpeptidase 3; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6469644
• Synonyms: Dpp3^-, Dpp3 KO
• Gene: Dpp3 Location: Chr19:4957257-4978315 bp, - strand Genetic Position: Chr19, 4.15 cM
• Alliance: Dpp3^{tm1d(EUCOMM)Hmgu} page
• IMPC: Dpp3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294242
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4970581 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4971737. The critical exon(s) is/are thus flanked by loxP sites (creating the Dpp3^{tm1c(EUCOMM)Hmgu} allele). The floxed targeted exons were deleted through cre-mediated recombination in mice carrying the tm1c allele, creating this tm1d knockout allele. Semi-quantitative RT-PCR on several tissues confirmed lack of transcript expression and Western blot analysis confirmed the absence of protein expression in total bone. (J:294242)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dpp3 Mutation: 39 strains or lines available

References

• Original: J:294242 Menale C, et al., Absence of Dipeptidyl Peptidase 3 Increases Oxidative Stress and Causes Bone Loss. J Bone Miner Res. 2019 Nov;34(11):2133-2148
• All: 1 reference(s)