Cep55^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cep55^{tm1c(EUCOMM)Hmgu}
• Name: centrosomal protein 55; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6458053
• Gene: Cep55 Location: Chr19:38043459-38062871 bp, + strand Genetic Position: Chr19, 32.69 cM, cytoband C3
• Alliance: Cep55^{tm1c(EUCOMM)Hmgu} page
• IMPC: Cep55 gene page

Mutation origin

• Mutant Cell Line: HEPD0726_6_A04
• Germline Transmission: Earliest citation of germline transmission: J:287102
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 38056153 of Chromosome 19 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 38057843. The critical exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287102)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cep55 Mutation: 71 strains or lines available

References

• Original: J:287102 Tedeschi A, et al., Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions. Nat Commun. 2020 Apr 8;11(1):1746
• All: 1 reference(s)