Gpr27^tm1Tac
Targeted Allele Detail

Summary

• Symbol: Gpr27^tm1Tac
• Name: G protein-coupled receptor 27; targeted mutation 1, Taconic
• MGI ID: MGI:6423080
• Gene: Gpr27 Location: Chr6:99669640-99672027 bp, + strand Genetic Position: Chr6, 46.22 cM, cytoband E1
• Alliance: Gpr27^tm1Tac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:286854
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The single coding exon was replaced with an IRES-lacZ-neomycin resistance cassette and a bovine growth hormone polyadenylation signal, followed by a PGK-puromycin resistance cassette. RT-qPCR analysis confirmed the absence of mRNA expression in the cerebral cortex and pancreatic islets isolated from homozygous mutant mice. Unexpectedly, Gpr27 deletion reduced expression of Eif4e3, a neighboring gene, likely by deleting transcription start sites on the anti-sense strand of the Gpr27 coding exon. (J:286854)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpr27 Mutation: 5 strains or lines available

References

• Original: J:286854 Chopra DG, et al., Deletion of Gpr27 in vivo reduces insulin mRNA but does not result in diabetes. Sci Rep. 2020 Mar 27;10(1):5629
• All: 1 reference(s)