Ifnlr1^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ifnlr1^{tm1b(EUCOMM)Wtsi}
• Name: interferon lambda receptor 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6416070
• Gene: Ifnlr1 Location: Chr4:135413598-135435492 bp, + strand Genetic Position: Chr4, 67.94 cM
• Alliance: Ifnlr1^{tm1b(EUCOMM)Wtsi} page
• IMPC: Ifnlr1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240624
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 135416791 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 135418289. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:240624)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifnlr1 Mutation: 36 strains or lines available

References

• Original: J:240624 Baldridge MT, et al., Expression of Ifnlr1 on Intestinal Epithelial Cells Is Critical to the Antiviral Effects of Interferon Lambda against Norovirus and Reovirus. J Virol. 2017 Apr 01;91(7)
• All: 7 reference(s)