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Gt(ROSA)26Sorem1(DPP4)Yowa
Endonuclease-mediated Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sorem1(DPP4)Yowa
Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Youchun Wang
MGI ID: MGI:6403442
Synonyms: Gt(ROSA)26Sortm1(SA-hDPP4-tdTomato), R26-hDPP4
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Gt(ROSA)26Sorem1(DPP4)Yowa expresses 1 gene
 
Mutation detailsCRISPR/Cas9 technology inserted the full-length human DPP4 cDNA linked to the red fluorescent protein tdTomato by an internal ribosomal entry site into the locus. Expression of the human cDNA is driven by the splice acceptor and A poly(A) sequence and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) were added to enhance RNA stability and translation efficiency. Western blot analysis of lung and brain tissue confirmed expression, with highest expression in the lung. (J:279060)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  540 strains or lines available
References
Original:  J:279060 Fan C, et al., A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV. Viruses. 2018 Aug 23;10(9)
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/10/2020
MGI 6.16
The Jackson Laboratory