Igs7^{tm2(tetO-tdTomato,-DTA*G128D)Rdiez}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm2(tetO-tdTomato,-DTA*G128D)Rdiez}
• Name: intergenic site 7; targeted mutation 2, Alberto Rosello Diez
• MGI ID: MGI:6389249
• Synonyms: Dragon-DTA, Igs7^TRE-LtSL-DTA
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm2(tetO-tdTomato,-DTA*G128D)Rdiez} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:290413
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The targeting vector was designed with, from 5' to 3', an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in the absence of transactivators), a Tet-response element/promoter (TRE tight, composed of seven TetO repeats and a minimal CMV promoter), a loxP-flanked red fluorescent protein variant (tdTomato) acting as a STOP cassette with 2 polyadenylation sequences (synthetic pA and hGHpA), a tox176 attenuated version of diphtheria toxin subunit alpha gene, a BGH polyA, a woodchuck virus RNA stabilizing sequence (WPRE), two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. G4 embryonic stem cells previously targeted with FRT3::AttB::PGK-neoR-polyA::FRT5::splice acceptor::3'hygro cassette::SV40 polyA:AttP in Igs7, were transfected with the replacement vector and Flp recombinase vector for recombinase-mediated cassette exchange (RCME). (J:290413)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 71 strains or lines available

References

• Original: J:290413 Ahmadzadeh E, et al., A collection of genetic mouse lines and related tools for inducible and reversible intersectional mis-expression. Development. 2020 May 28;147(10):dev186650
• All: 3 reference(s)