Ace^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace^em1Osb
• Name: angiotensin I converting enzyme; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6387031
• Synonyms: Ace-t^-37
• Gene: Ace Location: Chr11:105858774-105880790 bp, + strand Genetic Position: Chr11, 68.84 cM
• Alliance: Ace^em1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279308
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 37 bp deletion (5'-CTCCAGGACTGCCCAGCTTCCTCTTTCTGCTGCTCTG-3') in the 13th exon, disrupting testicular Ace which only transcribes from the 13th exon. The sgRNA sequence used for transfection were: 5'-GGCCAAGGTTGGGCTACTCC-3' and 5'-TCTTTCTGCTGCTCTGCTGT-3'. (J:279308)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ace Mutation: 54 strains or lines available

References

• Original: J:279308 Fujihara Y, et al., Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506
• All: 1 reference(s)