Otub1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Otub1^{tm1c(EUCOMM)Hmgu}
• Name: OTU domain, ubiquitin aldehyde binding 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6377208
• Synonyms: Otub1^f
• Gene: Otub1 Location: Chr19:7175571-7183649 bp, - strand Genetic Position: Chr19, 5.27 cM
• Alliance: Otub1^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Mutant Cell Line: HEPD0539_8_A05
• Germline Transmission: Earliest citation of germline transmission: J:280549
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 7181202 of Chromosome 19 upstream of the critical exons 2-3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2-3 at position 7182306. The critical exons 2 and 3 are thus flanked by loxP sites. This "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele, resulting in the removal of the lacZ and neomycin resistance gene and leaving exons 2-3 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:280549)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otub1 Mutation: 26 strains or lines available

References

• Original: J:280549 Li Y, et al., Preventing abnormal NF-kappaB activation and autoimmunity by Otub1-mediated p100 stabilization. Cell Res. 2019 Jun;29(6):474-485
• All: 3 reference(s)