Psma8^em1Yuc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Psma8^em1Yuc
• Name: proteasome subunit alpha 8; endonuclease-mediated mutation 1, Chao Yu
• MGI ID: MGI:6364865
• Gene: Psma8 Location: Chr18:14839208-14895358 bp, + strand Genetic Position: Chr18, 8.25 cM, cytoband A2
• Alliance: Psma8^em1Yuc page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination created a 5 bp deletion (TTGTG) in exon 2 resulting in a frameshift mutation and downstream premature stop codon. Western and immunoblot analyses confirmed the absence of expression in testes from homozygous males. (J:279342)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psma8 Mutation: 21 strains or lines available

References

• Original: J:279342 Zhang Q, et al., Meiosis I progression in spermatogenesis requires a type of testis-specific 20S core proteasome. Nat Commun. 2019 Jul 29;10(1):3387
• All: 1 reference(s)