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Knl1tm1c(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Knl1tm1c(EUCOMM)Hmgu
Name: kinetochore scaffold 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:6358098
Synonyms: Knl1fl
Gene: Knl1  Location: Chr2:118877600-118935982 bp, + strand  Genetic Position: Chr2, 59.85 cM, cytoband E5
Mutation
origin
Mutant Cell Line:  HEPD0665_2_E05
Germline Transmission:  Earliest citation of germline transmission: J:278483
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 119065470 of Chromosome 2 upstream of the critical exon 7 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 119066287. The critical exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:278483)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Knl1 Mutation:  65 strains or lines available
References
Original:  J:278483 Shi L, et al., Robust elimination of genome-damaged cells safeguards against brain somatic aneuploidy following Knl1 deletion. Nat Commun. 2019 Jun 13;10(1):2588
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory