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Opalintm1Tfr
Targeted Allele Detail
Nomenclature
Symbol: Opalintm1Tfr
Name: oligodendrocytic myelin paranodal and inner loop protein; targeted mutation 1, Teiichi Furuichi
MGI ID: MGI:6322964
Synonyms: Neo plus allele
Gene: Opalin  Location: Chr19:41061959-41077113 bp, - strand  Genetic Position: Chr19, 34.36 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:257142
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExons 2 - 6 were replaced by an FRT-flanked Pgk1 gene promoter-driven neomycin resistance gene (Pgk-neo) cassette. Western blot analysis revealed the complete absence of protein expression from myelin fractions of homozygous mutant brains at weaning, young adult and adult stages. Immunohistochemical analysis confirmed the absence of protein expression in the brains at 16 weeks of age. Expression of other tested major myelin components is similar to that in wild-type controls. (J:257142)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Opalin Mutation:  4 strains or lines available
References
Original:  J:257142 Yoshikawa F, et al., Mammalian-Specific Central Myelin Protein Opalin Is Redundant for Normal Myelination: Structural and Behavioral Assessments. PLoS One. 2016;11(11):e0166732
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2020
MGI 6.15
The Jackson Laboratory