B3glct^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: B3glct^{tm1d(KOMP)Wtsi}
• Name: beta-3-glucosyltransferase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6315841
• Synonyms: B3glct-delta4
• Gene: B3glct Location: Chr5:149601695-149686064 bp, + strand Genetic Position: Chr5, 89.18 cM
• Alliance: B3glct^{tm1d(KOMP)Wtsi} page
• IMPC: B3glct gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:286130
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 149632305 of Chromosome 5 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 149633181. The critical exon 4 is thus flanked by loxP sites.A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon 4. The knockout allele was created by deleting the cirtical exon 4 through subsequent cre-mediated recombination. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:286130)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any B3glct Mutation: 48 strains or lines available

References

• Original: J:286130 Holdener BC, et al., ADAMTS9 and ADAMTS20 are differentially affected by loss of B3GLCT in mouse model of Peters plus syndrome. Hum Mol Genet. 2019 Dec 15;28(24):4053-4066
• All: 1 reference(s)