B3glct^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: B3glct^{tm1b(KOMP)Wtsi}
• Name: beta-3-glucosyltransferase; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6274693
• Synonyms: B3glct-LacZ-delta4
• Gene: B3glct Location: Chr5:149601695-149686064 bp, + strand Genetic Position: Chr5, 89.18 cM
• Alliance: B3glct^{tm1b(KOMP)Wtsi} page
• IMPC: B3glct gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:286130
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 149632305 of Chromosome 5 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 149633181. The critical exon 4 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:94077)

Disease models

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any B3glct Mutation: 48 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 3 reference(s)