Tmem9^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem9^{tm1d(EUCOMM)Wtsi}
• Name: transmembrane protein 9; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6273211
• Synonyms: Tmem9^-, TMEM9 KO
• Gene: Tmem9 Location: Chr1:135935957-135962768 bp, + strand Genetic Position: Chr1, 59.51 cM
• Alliance: Tmem9^{tm1d(EUCOMM)Wtsi} page
• IMPC: Tmem9 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268691
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 135951901 of Chromosome 1 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 135952736. Exon 4 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:268691)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem9 Mutation: 12 strains or lines available

References

• Original: J:268691 Jung YS, et al., TMEM9 promotes intestinal tumorigenesis through vacuolar-ATPase-activated Wnt/beta-catenin signalling. Nat Cell Biol. 2018 Dec;20(12):1421-1433
• All: 2 reference(s)