5430431A17Rik^em1Doca
Endonuclease-mediated Allele Detail

Summary

• Symbol: 5430431A17Rik^em1Doca
• Name: RIKEN cDNA 5430431A17 gene; endonuclease-mediated mutation 1, Donal O'Carroll
• MGI ID: MGI:6256959
• Synonyms: Charme^-
• Gene: 5430431A17Rik Location: Chr7:44133941-44134837 bp, + strand Genetic Position: Chr7, Syntenic
• Alliance: 5430431A17Rik^em1Doca page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting introduced at the beginning of exon 2 a strong polyadenylation signal from beta-globin and two MAZ sites to promote efficient cleavage and transcriptional termination. Western blot analysis confirmed the absence of p and m isoforms. (J:266332)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any 5430431A17Rik Mutation: 0 strains or lines available

References

• Original: J:266332 Ballarino M, et al., Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice. EMBO J. 2018 Sep 14;37(18)
• All: 2 reference(s)