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P2rx7tm2.1(P2RX7*)Jde
Targeted Allele Detail
Nomenclature
Symbol: P2rx7tm2.1(P2RX7*)Jde
Name: purinergic receptor P2X, ligand-gated ion channel, 7; targeted mutation 1.1, Jan Deussing
MGI ID: MGI:6203043
Synonyms: P2rx7hQ460R
Gene: P2rx7  Location: Chr5:122643911-122691432 bp, + strand  Genetic Position: Chr5, 62.5 cM, cytoband F
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:251657
Parent Cell Line:  TBV2 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe targeting vector consists from 5' to 3': a loxP site followed by the 3'-end (1.4-kb) of mouse intron 1; the murine exon 2 replaced by the human P2RX7 cDNA comprising exons 2-13 with the Gln460Arg variant; a reverse oriented selection marker flanked by frt sites which consists of a phosphoglycerate kinase (PGK) promoter driven neomycin resistance gene equipped with a bovine growth hormone (bGH) poly A signal (pA), a second loxP site and a quadruple poly A signal consisting of a bGHpA, a PGK pA, and 2 SV40 pAs. After germ-line transmission of the modified allele via homologous recombination in ES cells, offspring from male chimeras are bred. The FRT flanked neo cassette was removed by breeding to deleter-flp mice. (J:251657)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any P2rx7 Mutation:  15 strains or lines available
References
Original:  J:251657 Metzger MW, et al., Heterozygosity for the Mood Disorder-Associated Variant Gln460Arg Alters P2X7 Receptor Function and Sleep Quality. J Neurosci. 2017 Nov 29;37(48):11688-11700
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/15/2019
MGI 6.14
The Jackson Laboratory