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P2rx7tm1.1(P2RX7)Jde
Targeted Allele Detail
Nomenclature
Symbol: P2rx7tm1.1(P2RX7)Jde
Name: purinergic receptor P2X, ligand-gated ion channel, 7; targeted mutation 1.1, Jan Deussing
MGI ID: MGI:6203041
Synonyms: P2rx7hP2RX7, P2rx7hWT
Gene: P2rx7  Location: Chr5:122643911-122691432 bp, + strand  Genetic Position: Chr5, 62.5 cM, cytoband F
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:244726
Parent Cell Line:  TBV2 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout)
Mutations:    Insertion, Intergenic deletion
 
Mutation detailsThe targeting vector consists from 5' to 3': a loxP site followed by the 3'-end (1.4-kb) of mouse intron 1; the murine exon 2 replaced by the human P2RX7 cDNA comprising exons 2-13; a reverse oriented selection marker flanked by frt sites which consists of a phosphoglycerate kinase (PGK) promoter driven neomycin resistance gene equipped with a bovine growth hormone (bGH) poly A signal (pA), a second loxP site and a quadruple poly A signal consisting of a bGHpA, a PGK pA, and 2 SV40 pAs. After germ-line transmission of the modified allele via homologous recombination in ES cells, offspring from male chimeras are bred. The FRT flanked neo cassette was removed by breeding to deleter-flp mice. (J:244726)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any P2rx7 Mutation:  15 strains or lines available
References
Original:  J:244726 Metzger MW, et al., Genetically dissecting P2rx7 expression within the central nervous system using conditional humanized mice. Purinergic Signal. 2017 Jun;13(2):153-170
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory