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U2af1tm1.1Hev
Targeted Allele Detail
Summary
Symbol: U2af1tm1.1Hev
Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1; targeted mutation 1.1, Harold E Varmus
MGI ID: MGI:6202047
Synonyms: MGS34F
Gene: U2af1  Location: Chr17:31866055-31877866 bp, - strand  Genetic Position: Chr17, 17.02 cM
Alliance: U2af1tm1.1Hev page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:267082
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Humanized sequence)
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsThe targeting vector is designed with a loxP site downstream of the endogenous exon 1 of the gene followed by exons 2-8. A transcriptional STOP sequence (3 repeats of SV40 late poly A sequence) followed by an FRT-flanked neomycin selection cassette and a second loxP site were inserted downstream of the exon 2-8 sequence of the targeting construct. A missense mutation (TCT > TTT) was created in the endogenous exon 2, resulting in a serine to phenylalanine mutation at position 34 (S34F). (J:267082)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any U2af1 Mutation:  33 strains or lines available
Notes
ES cell line = HG3 - (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1
References
Original:  J:267082 Fei DL, et al., Impaired hematopoiesis and leukemia development in mice with a conditional knock-in allele of a mutant splicing factor gene U2af1. Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):E10437-E10446
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory