Katnal2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Katnal2^{tm1c(EUCOMM)Wtsi}
• Name: katanin p60 subunit A-like 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6164199
• Synonyms: Katnal2^Flox
• Gene: Katnal2 Location: Chr18:77064844-77135004 bp, - strand Genetic Position: Chr18, 52.02 cM, cytoband E3
• Alliance: Katnal2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Katnal2 gene page

Spermatogenic defects in Katnal2^{tm1a(EUCOMM)Wtsi}/Katnal2^{tm1a(EUCOMM)Wtsi} Tg(Stra8-icre)1Reb/0 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0656_4_A03
• Germline Transmission: Earliest citation of germline transmission: J:250115
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 77104991 of Chromosome 18 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 77105793. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exon 3 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:250115)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Katnal2 Mutation: 199 strains or lines available

References

• Original: J:250115 Dunleavy JEM, et al., Katanin-like 2 (KATNAL2) functions in multiple aspects of haploid male germ cell development in the mouse. PLoS Genet. 2017 Nov;13(11):e1007078
• All: 1 reference(s)