Qrfp^tm1Stak
Targeted Allele Detail

Summary

• Symbol: Qrfp^tm1Stak
• Name: pyroglutamylated RFamide peptide; targeted mutation 1, Takeshi Sakurai
• MGI ID: MGI:6163999
• Synonyms: Qrfp^-
• Gene: Qrfp Location: Chr2:31696180-31700592 bp, - strand Genetic Position: Chr2, 21.89 cM
• Alliance: Qrfp^tm1Stak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:250913
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region of the prepro-QRFP sequence in exon 2 of the gene was replaced with an enhanced GFP sequence and a pgk-Neo selection cassette. GFP fluorescence was detected in QRFP neurons without any ectopic expression. Immunohistochemistry and in situ hybridization of brain coronal sections confirmed the complete absence of protein expression in homozygous mutant mice. (J:250913)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Qrfp Mutation: 11 strains or lines available

References

• Original: J:250913 Okamoto K, et al., QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior. PLoS One. 2016;11(11):e0164716
• All: 1 reference(s)