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Pikfyvetm1b(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Pikfyvetm1b(EUCOMM)Hmgu
Name: phosphoinositide kinase, FYVE type zinc finger containing; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:6156635
Synonyms: PIKfyveflox
Gene: Pikfyve  Location: Chr1:65186683-65278695 bp, + strand  Genetic Position: Chr1, 32.94 cM, cytoband C3
Mutation
origin
Mutant Cell Lines:  HEPD0682_4_B04, HEPD0682_4_D04
Germline Transmission:  Earliest citation of germline transmission: J:244776
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 65196033 of Chromosome 1 upstream of the critical exon 6 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 65197072. The critical exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:244776)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pikfyve Mutation:  5 strains or lines available
Notes
Cell line HEPD0682_4_B04 and HEPD0682_4_D04 were successfully used to make chimeric mice. Germline transmission was accomplished from a mixed population. J:244776
References
Original:  J:244776 Kawasaki T, et al., Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice. EMBO J. 2017 Jun 14;36(12):1707-1718
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/31/2020
MGI 6.15
The Jackson Laboratory