Samd7^tm1Tfur
Targeted Allele Detail

Summary

• Symbol: Samd7^tm1Tfur
• Name: sterile alpha motif domain containing 7; targeted mutation 1, Takahisa Furukawa
• MGI ID: MGI:6150316
• Gene: Samd7 Location: Chr3:30800481-30821323 bp, + strand Genetic Position: Chr3, 14.27 cM, cytoband A3
• Alliance: Samd7^tm1Tfur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:253631
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4-6 were replaced with a loxP site flanked neomycin selection cassette. This is predicted to cause a a premature stop codon due to a frame shift, resulting in a truncated peptide that would lack the SAM domain. Absence of mRNA and protein expression from this allele in homozygous mice was confirmed by RT-PCR and Western blot, respectively. (J:253631)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Samd7 Mutation: 24 strains or lines available

References

• Original: J:253631 Omori Y, et al., Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity. Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8264-E8273
• All: 2 reference(s)