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Spata16em2Osb
Endonuclease-mediated Allele Detail
Nomenclature
Symbol: Spata16em2Osb
Name: spermatogenesis associated 16; endonuclease mediated mutation 2, Research Institute for Microbial Diseases, Osaka U
MGI ID: MGI:6147690
Synonyms: Spata16-781
Gene: Spata16  Location: Chr3:26637620-26983212 bp, + strand  Genetic Position: Chr3, 10.74 cM, cytoband A3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:258202
Parent Cell Line:  EGR-G01 (ES Cell)
Strain of Origin:  (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsCRISPR mutagenesis was used to generate a 781 bp deletion that removed 88 bp out of 90 bp of exon 4 which encodes the C-terminus of the tetratricopeptide repeat (TPR) domain. This deletion resulted in the mistranslation of the remaining 2 bp of the mutant fourth exon. (J:258202)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Spata16 Mutation:  5 strains or lines available
Notes
EGR-G01 cells carry one copy of Tg(CAG-EGFP,Acr-EGFP)2Osb.
References
Original:  J:258202 Fujihara Y, et al., Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models. Int J Mol Sci. 2017 Oct 21;18(10)
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/24/2020
MGI 6.15
The Jackson Laboratory