Ptma^tm1.1Got
Targeted Allele Detail

Summary

• Symbol: Ptma^tm1.1Got
• Name: prothymosin alpha; targeted mutation 1.1, Otsuka GEN Research Institute
• MGI ID: MGI:6121068
• Synonyms: ProTalpha^-
• Gene: Ptma Location: Chr1:86454448-86458434 bp, + strand Genetic Position: Chr1, 43.96 cM, cytoband C5
• Alliance: Ptma^tm1.1Got page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240549
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and a loxP-flanked MC1-neo cassette was inserted downstream of exon 3. Cre-mediated recombination deleted exons 2-3 and the MC1-neo cassette. Real-time RT-PCR analysis revealed significantly reduced mRNA levels in heterozygous hippocampal dentate gyrus and CA1 regions. Western blot analysis of heterozygous brain tissue confirmed a significant reduction in protein levels, with 73.8% of wild-type levels in the olfactory bulb, 32.1% in anterior cingulate cortex, 37.7% in motor cortex, 30.5% in somatosensory cortex, 44.9% in caudate-putamen (striatum), 43.8% in amygdala, 57.8% in hippocampus, and 47.4% in cerebellum. (J:240549)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptma Mutation: 20 strains or lines available

References

• Original: J:240549 Ueda H, et al., Prothymosin alpha-deficiency enhances anxiety-like behaviors and impairs learning/memory functions and neurogenesis. J Neurochem. 2017 Apr;141(1):124-136
• All: 1 reference(s)