Atp4a^tm1.1Ocalv
Targeted Allele Detail

Summary

• Symbol: Atp4a^tm1.1Ocalv
• Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide; targeted mutation 1.1, Oriol Calvete
• MGI ID: MGI:6118153
• Synonyms: ATP4a^R703C, Atp4a^tm1.1Sgo
• Gene: Atp4a Location: Chr7:30411634-30424959 bp, + strand Genetic Position: Chr7, 19.2 cM
• Alliance: Atp4a^tm1.1Ocalv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235360
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 14 was replaced with a modified exon 14 in with CGA was mutated to TGC at position 709 resulting in substitution of cysteine to arginine and a silent mutation in which CTG was mutated to CTT in codon 709 (to generate a HindIII site). A FRT-flanked PGK/bg2-neo resistance cassette was introduced into the intron preceding 5' to exon 13, with a transcriptional orientation opposite to that of the gene. Flp-mediated recombination removed the selection cassette. This mutation corresponds to the human p.R703C mutation responsible for atypical type I familial gastric neuroendocrine tumors. (J:235360)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp4a Mutation: 63 strains or lines available

References

• Original: J:235360 Calvete O, et al., A knockin mouse model for human ATP4aR703C mutation identified in familial gastric neuroendocrine tumors recapitulates the premalignant condition of the human disease and suggests new therapeutic strategies. Dis Model Mech. 2016 Sep 1;9(9):975-84
• All: 2 reference(s)