Crtc2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Crtc2^{tm1c(EUCOMM)Wtsi}
• Name: CREB regulated transcription coactivator 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6111714
• Synonyms: Crtc2 flox
• Gene: Crtc2 Location: Chr3:90161475-90171432 bp, + strand Genetic Position: Chr3, 39.21 cM, cytoband F2
• Alliance: Crtc2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Crtc2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251811
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 90165282 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 90166104. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:251811)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crtc2 Mutation: 27 strains or lines available

References

• Original: J:251811 Han HS, et al., Hepatic Crtc2 controls whole body energy metabolism via a miR-34a-Fgf21 axis. Nat Commun. 2017 Nov 30;8(1):1878
• All: 3 reference(s)