Col1a1^{tm2(tetO-GFP/RNAi:Smc1a)Iaai}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(tetO-GFP/RNAi:Smc1a)Iaai}
• Name: collagen, type I, alpha 1; targeted mutation 2, Iannis Aifantis
• MGI ID: MGI:5911565
• Synonyms: Smc1a^(shRNA)
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(tetO-GFP/RNAi:Smc1a)Iaai} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229031
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm2(tetO-GFP/RNAi:Smc1a)Iaai} involves 1 genes/genome features (Smc1a) View all
• Mutation details: A construct containing a tetracycline promoter and a GFP fusion with a Mir30-based short hairpin sequence targeting Smc1a (Smc1a-3921) was inserted in the 3' UTR. Following tetracycline induced expression of the RNAi expression of Smc1a is strongly reduced and expression of associated protein members of the cohesin complex are also reduced. (J:229031)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

References

• Original: J:229031 Mullenders J, et al., Cohesin loss alters adult hematopoietic stem cell homeostasis, leading to myeloproliferative neoplasms. J Exp Med. 2015 Oct 19;212(11):1833-50
• All: 1 reference(s)