Rorb^tm2.1Df
Targeted Allele Detail

Summary

• Symbol: Rorb^tm2.1Df
• Name: RAR-related orphan receptor beta; targeted mutation 2.1, Douglas Forrest
• MGI ID: MGI:5905301
• Synonyms: Rorb^2lacz, Rorb^2z
• Gene: Rorb Location: Chr19:18907969-19088560 bp, - strand Genetic Position: Chr19, 13.28 cM
• Alliance: Rorb^tm2.1Df page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242481
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s), Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ reporter replaced the 3' end of exon encoding the N-terminus of isoform 2. Cre-mediated recombination removed the selection cassette. RT-PCR confirmed the absence of transcripts for isoform 2 but expression of isoform 1 transcript. (J:242481)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rorb Mutation: 38 strains or lines available

References

• Original: J:242481 Fu Y, et al., Feedback induction of a photoreceptor-specific isoform of retinoid-related orphan nuclear receptor beta by the rod transcription factor NRL. J Biol Chem. 2014 Nov 21;289(47):32469-80
• All: 2 reference(s)