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Mpc1tm1c(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Mpc1tm1c(EUCOMM)Wtsi
Name: mitochondrial pyruvate carrier 1; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID: MGI:5903307
Synonyms: Mcp1fl
Gene: Mpc1  Location: Chr17:8502590-8516499 bp, + strand  Genetic Position: Chr17, 4.92 cM
Alliance: Mpc1tm1c(EUCOMM)Wtsi page
IMPC: Mpc1 gene page
Mutation
origin
Mutant Cell Line:  EPD0377_6_F04
Germline Transmission:  Earliest citation of germline transmission: J:236662
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready)
Mutation:    Insertion     Vector: L1L2_Pgk_P
 
Mutation detailsThe L1L2_Pgk_P cassette was inserted at position 8514918 of Chromosome 17 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 8516753. The critical exons 3 through 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:236662)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 6 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mpc1 Mutation:  13 strains or lines available
References
Original:  J:236662 Gray LR, et al., Hepatic Mitochondrial Pyruvate Carrier 1 Is Required for Efficient Regulation of Gluconeogenesis and Whole-Body Glucose Homeostasis. Cell Metab. 2015 Oct 6;22(4):669-81
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory