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Igsf11tm1d(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: Igsf11tm1d(KOMP)Wtsi
Name: immunoglobulin superfamily, member 11; targeted mutation 1d, Wellcome Trust Sanger Institute
MGI ID: MGI:5897622
Gene: Igsf11  Location: Chr16:38713033-38847521 bp, + strand  Genetic Position: Chr16, 26.87 cM
Alliance: Igsf11tm1d(KOMP)Wtsi page
IMPC: Igsf11 gene page
Mutation
origin
Mutant Cell Line:  EPD0297_2_A07
Germline Transmission:  Earliest citation of germline transmission: J:234439
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 38828713 of Chromosome 16 upstream of the critical exons 3 through 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3 through 5 at position 38829597. The critical exons 3 through 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in the brain. (J:234439)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Igsf11 Mutation:  33 strains or lines available
Notes
Cell line EPD0297_2_A07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:234439
References
Original:  J:234439 Jang S, et al., Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity. Nat Neurosci. 2016 Jan;19(1):84-93
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory