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Anks1btm1b(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Anks1btm1b(KOMP)Wtsi
Name: ankyrin repeat and sterile alpha motif domain containing 1B; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:5896631
Gene: Anks1b  Location: Chr10:89873509-90973300 bp, + strand  Genetic Position: Chr10, 45.28 cM
Mutation
origin
Mutant Cell Line:  EPD0613_3_C08
Germline Transmission:  Earliest citation of germline transmission: J:240601
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 90680338 of Chromosome 10 upstream of exon 17 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 17 at position 90681189. Exon 17 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked exon 17. Western blot analysis of cortical tissue lysates from homozygous mice confirmed the absence of protein expression. (J:240601)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Anks1b Mutation:  2 strains or lines available
References
Original:  J:240601 Enga RM, et al., Initial characterization of behavior and ketamine response in a mouse knockout of the post-synaptic effector gene Anks1b. Neurosci Lett. 2017 Feb 22;641:26-32
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/26/2019
MGI 6.14
The Jackson Laboratory