Anxa10^em1Siag
Endonuclease-mediated Allele Detail

Summary

• Symbol: Anxa10^em1Siag
• Name: annexin A10; endonuclease-mediated mutation 1, Shinji Sasaki
• MGI ID: MGI:5888868
• Synonyms: Anxa10^-
• Gene: Anxa10 Location: Chr8:62510076-62576184 bp, - strand Genetic Position: Chr8, 31.55 cM
• Alliance: Anxa10^em1Siag page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-Cas9 methodology generated an 8-bp deletion (74-81 bp) in exon 2 of the gene that was predicted to cause a frame-shift and generate terminal codons at multiple positions. Western blot and immunohistochemistry experiments showed that no encoded protein was present in tissues of homozygous mutant embryos. (J:239403)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Anxa10 Mutation: 28 strains or lines available

References

• Original: J:239403 Sasaki S, et al., Loss of maternal ANNEXIN A10 via a 34-kb deleted-type copy number variation is associated with embryonic mortality in Japanese Black cattle. BMC Genomics. 2016 Nov 24;17(1):968
• All: 1 reference(s)