Mir196b^tm1.1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir196b^tm1.1Wtsi
• Name: microRNA 196b; targeted mutation 1.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5805882
• Synonyms: Mir196b^{tm1.1(mirKO)Wtsi}
• Gene: Mir196b Location: Chr6:52207061-52207145 bp, - strand Genetic Position: Chr6, 25.4 cM
• Alliance: Mir196b^tm1.1Wtsi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226712
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 436 starting at position 52159915 and ending at position 52159479 of Chromosome 6 (Genome Build 37). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. Cre-mediated recombination removed the selection cassette. (J:226712)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir196b Mutation: 2 strains or lines available

References

• Original: J:226712 Wong SF, et al., Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs. Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4884-93
• All: 2 reference(s)