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Aspmtm1(cre)Mrc
Targeted Allele Detail
Nomenclature
Symbol: Aspmtm1(cre)Mrc
Name: abnormal spindle microtubule assembly; targeted mutation 1, Mario R Capecchi
MGI ID: MGI:5753079
Synonyms: APSMdelta
Gene: Aspm  Location: Chr1:139454772-139494091 bp, + strand  Genetic Position: Chr1, 61.45 cM, cytoband F
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:228026
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsThe majority of exon 18 was replaced with an ires-cre cassette followed by a CAG-driven FRT-flanked neo gene via homologous recombination. The mutant protein lacks the terminal 1717 amino acids containing the calmodulin binding domain and the C-terminus. The deletion of a 2.6-kb genomic region of exon 18 yields a nonsense mutation resulting in a bicistronic mRNA product containing protein truncated at amino acid 1405, and ires-derived cre expression. Northern and Western blot analysis confirmed the predicted truncation of the mRNA and protein in homozygotes. (J:228026)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Aspm     Summary of all recombinase alleles driven by Aspm.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Aspm Mutation:  3 strains or lines available
References
Original:  J:228026 Capecchi MR, et al., ASPM regulates symmetric stem cell division by tuning Cyclin E ubiquitination. Nat Commun. 2015;6:8763
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/01/2019
MGI 6.14
The Jackson Laboratory