Cdk4^tm2.2Bbd
Targeted Allele Detail

Summary

• Symbol: Cdk4^tm2.2Bbd
• Name: cyclin dependent kinase 4; targeted mutation 2.2, Mariano Barbacid
• MGI ID: MGI:5705761
• Synonyms: Cdk4^frt
• Gene: Cdk4 Location: Chr10:126899404-126903157 bp, + strand Genetic Position: Chr10, 74.5 cM, cytoband D3
• Alliance: Cdk4^tm2.2Bbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: An frt site was inserted 230 bp upstream of exon 2 and a frt-flanked hygromycin resistance cassette 115 bp downstream of exon 4 in ES cells already carrying the Cdk2^tm2.1Sgo conditional mutation of Cdk2 and integrated into the same chromosome. The selection cassette was excised by Flp-mediated recombination, leaving exons 2-4 flanked by frt sites. (J:125217)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk4 Mutation: 57 strains or lines available

References

• Original: J:125217 Barriere C, et al., Mice thrive without Cdk4 and Cdk2. Mol Oncol. 2007 Jun;1(1):72-83
• All: 3 reference(s)