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Brpf1tm1d(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Brpf1tm1d(EUCOMM)Wtsi
Name: bromodomain and PHD finger containing, 1; targeted mutation 1d, Wellcome Trust Sanger Institute
MGI ID: MGI:5705132
Synonyms: Brpf1-
Gene: Brpf1  Location: Chr6:113284098-113301821 bp, + strand  Genetic Position: Chr6, 52.75 cM, cytoband E3
Mutation
origin
Mutant Cell Lines:  EPD0069_1_G04, EPD0069_1_G06
Germline Transmission:  Earliest citation of germline transmission: J:222304
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_gt2
 
Mutation detailsThe L1L2_gt2 cassette was inserted at position 113316107 of Chromosome 6 upstream of the critical exons 4 through 6 (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 4-6 at position 113317975. The critical exons 4 through 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:222304)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Brpf1 Mutation:  24 strains or lines available
Notes
Cell line EPD0069_1_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:222304
References
Original:  J:222304 You L, et al., Expression atlas of the multivalent epigenetic regulator Brpf1 and its requirement for survival of mouse embryos. Epigenetics. 2014 Jun;9(6):860-72
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/18/2022
MGI 6.17
The Jackson Laboratory