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Targeted Allele Detail
Symbol: Brpf1tm1d(EUCOMM)Wtsi
Name: bromodomain and PHD finger containing, 1; targeted mutation 1d, Wellcome Trust Sanger Institute
MGI ID: MGI:5705132
Synonyms: Brpf1-
Gene: Brpf1  Location: Chr6:113284098-113301821 bp, + strand  Genetic Position: Chr6, 52.75 cM, cytoband E3
Mutant Cell Lines:  EPD0069_1_G04, EPD0069_1_G06
Germline Transmission:  Earliest citation of germline transmission: J:222304
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_gt2
Mutation detailsThe L1L2_gt2 cassette was inserted at position 113316107 of Chromosome 6 upstream of the critical exons 4 through 6 (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 4-6 at position 113317975. The critical exons 4 through 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:222304)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Brpf1 Mutation:  24 strains or lines available
Cell line EPD0069_1_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:222304
Original:  J:222304 You L, et al., Expression atlas of the multivalent epigenetic regulator Brpf1 and its requirement for survival of mouse embryos. Epigenetics. 2014 Jun;9(6):860-72
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory