Vcam1^tm2Dmil
Targeted Allele Detail

Summary

• Symbol: Vcam1^tm2Dmil
• Name: vascular cell adhesion molecule 1; targeted mutation 2, David Milstone
• MGI ID: MGI:5700541
• Synonyms: Vcam^kB
• Gene: Vcam1 Location: Chr3:115903669-115923337 bp, - strand Genetic Position: Chr3, 50.17 cM
• Alliance: Vcam1^tm2Dmil page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227109
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Sequential tag and exchange gene targeting was used to introduce a 6 nucleotide mutation in the 5NF-B binding site of the transcriptional promoter, and a 3 nucleotide mutation in the 5UTR of Vcam1 in exon 1. The first, "tag", targeting vector replaces exon 1 (encoding the signal peptide) and part of exon 2 (encoding immunoglobulin-like domain 1) with a pgk-neomycin/TK cassettes. The Tag construct is transfected into embryonic stems cells and a single correctly targeted clone is transfected with the second construct. The second "Exchange" targeting construct replaces the neo cassette and introduces a 6 nucleotide substitution 5' of the transcription start site resulting in the disruption of the 5'NFKB1 (NF-kappaB) binding site domain and a 3 nucleotide substitution downstream of the translation start site in the 5' UTR of exon 1. The 3 nt substitution is used to distinguish the mutant and wild-type mRNA and hnRNA. Western blot ananlysis demonstrates the presence of mutant protein in heart endothelial cells. (J:227109)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vcam1 Mutation: 42 strains or lines available

References

• Original: J:227109 Milstone DS, et al., Differential role of an NF-kappaB transcriptional response element in endothelial versus intimal cell VCAM-1 expression. Circ Res. 2015 Jul 3;117(2):166-77
• All: 1 reference(s)