Fancb^em1Shnk
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fancb^em1Shnk
• Name: Fanconi anemia, complementation group B; endonuclease-mediated mutation 1, Satoshi H Namekawa
• MGI ID: MGI:5688399
• Gene: Fancb Location: ChrX:163763678-163780266 bp, + strand Genetic Position: ChrX, 76.75 cM
• Alliance: Fancb^em1Shnk page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Zinc finger nuclease technology was used to modify exon 2 by deleting CAGTT. This mutation results in a premature stop codon and truncation at amino acid 307 of 853. Western blot analysis confirmed the absence of protein in testes. The allele was isolated in line 84. (J:224588)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fancb Mutation: 3 strains or lines available

Notes

Lines 164, 167, 171 and 84 were generated in the same experiment. However, lines 164, 167 and 171 were all infertile males. J:224588

References

• Original: J:224588 Kato Y, et al., FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis. Hum Mol Genet. 2015 Sep 15;24(18):5234-49
• All: 3 reference(s)