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Nlgn3tm4.1Sud
Targeted Allele Detail
Nomenclature
Symbol: Nlgn3tm4.1Sud
Name: neuroligin 3; targeted mutation 4.1, Thomas C Sudhof
MGI ID: MGI:5649372
Synonyms: NL3-cKO, NL3fl
Gene: Nlgn3  Location: ChrX:101299179-101321350 bp, + strand  Genetic Position: ChrX, 43.95 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:214636
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready)
Mutation:    Insertion
 
Mutation detailsThe following elements were inserted into intron 1: an FRT site, a duplicate of the 5' end of the ATG translation start site-containing exon 2, a full-length Nlgn3 cDNA (incorporating mutations corresponding to Nlgn1 "5/32" (L399A, N400A, D402N, E297A, and K306A) that abolish neurexin binding) fused to the cytoplasmic monomeric green fluorescent protein mVenus gene (lacking splice site A), an F3 FRT site, a neomycin resistance gene cassette, a tandem FRT/F3 FRT site, and a loxP511 site. A second loxP511 site was inserted into intron 3 to allow for conditional cre-mediated deletion of exons 2 and 3. This conditional knockout allele, expressing the wild-type peptide, was created through subsequent flp-mediated recombination via the FRT sites (which removes the the neo cassette and knockin transcript and leaves exons 2-3 floxed). (J:214636)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nlgn3 Mutation:  21 strains or lines available
References
Original:  J:214636 Rothwell PE, et al., Autism-associated neuroligin-3 mutations commonly impair striatal circuits to boost repetitive behaviors. Cell. 2014 Jul 3;158(1):198-212
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/17/2019
MGI 6.14
The Jackson Laboratory