Nlgn3^tm4.1Sud
Targeted Allele Detail

Summary

• Symbol: Nlgn3^tm4.1Sud
• Name: neuroligin 3; targeted mutation 4.1, Thomas C Sudhof
• MGI ID: MGI:5649372
• Synonyms: NL3-cKO, NL3^fl
• Gene: Nlgn3 Location: ChrX:100342785-100364956 bp, + strand Genetic Position: ChrX, 43.95 cM
• Alliance: Nlgn3^tm4.1Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214636
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The following elements were inserted into intron 1: an FRT site, a duplicate of the 5' end of the ATG translation start site-containing exon 2, a full-length Nlgn3 cDNA (incorporating mutations corresponding to Nlgn1 "5/32" (L399A, N400A, D402N, E297A, and K306A) that abolish neurexin binding) fused to the cytoplasmic monomeric green fluorescent protein mVenus gene (lacking splice site A), an F3 FRT site, a neomycin resistance gene cassette, a tandem FRT/F3 FRT site, and a loxP511 site. A second loxP511 site was inserted into intron 3 to allow for conditional cre-mediated deletion of exons 2 and 3. This conditional knockout allele, expressing the wild-type peptide, was created through subsequent flp-mediated recombination via the FRT sites (which removes the the neo cassette and knockin transcript and leaves exons 2-3 floxed). (J:214636)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nlgn3 Mutation: 35 strains or lines available

References

• Original: J:214636 Rothwell PE, et al., Autism-associated neuroligin-3 mutations commonly impair striatal circuits to boost repetitive behaviors. Cell. 2014 Jul 3;158(1):198-212
• All: 16 reference(s)