Tg(Tcra6C2,Tcrb6C2)16Chi
Transgene Detail

Summary

• Symbol: Tg(Tcra6C2,Tcrb6C2)16Chi
• Name: transgene insertion 16, Francis Chisari
• MGI ID: MGI:5648206
• Synonyms: Env28 TCR transgenic
• Transgene: Tg(Tcra6C2,Tcrb6C2)16Chi Location: unknown
• Alliance: Tg(Tcra6C2,Tcrb6C2)16Chi page

Transgene origin

• Strain of Origin: BALB/cByJ or C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tcra6C2,Tcrb6C2)16Chi expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Rearranged Valpha4.1 JalphaNEW and Vbeta1.1 Jbeta2.5 sequences are obtained from L-restricted CD3<+> CD4<-> CD8<+> cytotoxic T lymphocytes (clone 6C2), which recognizes an epitope located between residues 28-39 of HBsAg. These TCR sequences were cloned into TCR cassette expression vectors (pTalpha and pTbeta), and co-injected. The primary investigator indicates that cells from founder lines 16 and 36 exhibit no differences in epitope recognition. (J:213364)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

TCR expression cassettes into which the TCRalpha and TCRbeta cDNA sequences had been cloned were co-injected into (BALB/cByJ x C57BL/6J)F2 fertilized oocytes.

References

• Original: J:213364 Isogawa M, et al., CD40 activation rescues antiviral CD8(+) T cells from PD-1-mediated exhaustion. PLoS Pathog. 2013;9(7):e1003490
• All: 5 reference(s)