Igs2tm1.1(CAG-cas9*)Mmw
Targeted Allele Detail
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Symbol: |
Igs2tm1.1(CAG-cas9*)Mmw |
Name: |
intergenic site 2; targeted mutation 1.1, Monte Winslow |
MGI ID: |
MGI:5645518 |
Synonyms: |
H11Cas9 |
Gene: |
Igs2 Location: unknown Genetic Position: Chr11, Syntenic
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Alliance: |
Igs2tm1.1(CAG-cas9*)Mmw page
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Allele Type: |
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Targeted (Endonuclease) |
Mutation: |
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Insertion
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Mutation details: The targeting construct contains a full-length attB site, the CMV enhancer/chicken beta-actin core promoter (CAGGS), a loxP-STOP(3xSV40 polyA)- loxP cassette (LSL), a 3xFLAG sequence, a nuclear localization signal (NLS), a human codon-optimized S. pyogenes cas9 gene (hSpCas9), a second NLS and the SV40 polyA signal. The embryonic stem cells used were previously targeted with the H11 allele ( Igs2tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. Cre-mediated recombination removed the LSL cassette.
(J:222466)
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Original: |
J:222466 Chiou SH, et al., Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015 Jul 15;29(14):1576-85 |
All: |
13 reference(s) |
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