Tg(HLA-DRA,HLA-DRB5*0101)hiKito
Transgene Detail

Summary

• Symbol: Tg(HLA-DRA,HLA-DRB5*0101)hiKito
• Name: transgene insertion hi, Kouichi Ito
• MGI ID: MGI:5635424
• Synonyms: DR2a^hi
• Transgene: Tg(HLA-DRA,HLA-DRB5*0101)hiKito Location: unknown
• Alliance: Tg(HLA-DRA,HLA-DRB5*0101)hiKito page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(HLA-DRA,HLA-DRB5*0101)hiKito expresses 4 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  mouse

  H2-Ea (MGI:95900)

  Expresses a human HLA-DRA and HLA-DRB5*01:01 fused to mouse H2-Ea-ps and H2-EB1

  mouse

  H2-Eb1 (MGI:95901)

  Expresses a human HLA-DRA and HLA-DRB5*01:01 fused to mouse H2-Ea-ps and H2-EB1

  human

  HLA-DRA (3122)

  H2-Ea

  (MGI:95900)

  Expresses a human HLA-DRA and HLA-DRB5*01:01 fused to mouse H2-Ea-ps and H2-EB1

  human

  HLA-DRB5 (3127)

  H2-Eb1	(MGI:95901)
  H2-Eb2	(MGI:95902)

  Expresses a human HLA-DRA and HLA-DRB5*01:01 fused to mouse H2-Ea-ps and H2-EB1

• Mutation details: A chimeric MHC class II complex was created by fusing the human peptide antigen binding domains of class II alpha and beta chains (HLA-DRA and HLA-DRB5*01:01) to the remaining domains of murine class II alpha or beta chains (H2-Ea-ps and H2-Eb1 genes that make up mouse I-Ed). The chimeric gene constructs were manipulated by replacing exon 2 of IE^d-alpha or IE^d-beta genomic DNA with gene fragments containing exon 2 of HLA-DRA or HLA-DRB5*01:01, respectively. The native murine promoters were left intact. Two lines were created, a high expressing line (hi) and a low expressing line (lo). (J:189816)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:189816 Quandt JA, et al., Myelin basic protein-specific TCR/HLA-DRB5*01:01 transgenic mice support the etiologic role of DRB5*01:01 in multiple sclerosis. J Immunol. 2012 Sep 15;189(6):2897-908
• All: 2 reference(s)