Chrna7^tm1.1Ehs
Targeted Allele Detail

Summary

• Symbol: Chrna7^tm1.1Ehs
• Name: cholinergic receptor, nicotinic, alpha polypeptide 7; targeted mutation 1.1, National Institute of Environmental Health Science
• MGI ID: MGI:5620283
• Synonyms: alpha7^flox, alpha7nAChR^flox
• Gene: Chrna7 Location: Chr7:62748440-62862274 bp, - strand Genetic Position: Chr7, 34.47 cM
• Alliance: Chrna7^tm1.1Ehs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219327
• Parent Cell Line: ART B6-3, B6-3 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The targeting vector contains a loxP site 290 bp upstream of exon 4 and loxP site followed by a FRT-flanked PGK-Neo selection cassette 380 bp downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 4 floxed. (J:219327)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna7 Mutation: 49 strains or lines available

References

• Original: J:219327 Hernandez CM, et al., Research tool: Validation of floxed alpha7 nicotinic acetylcholine receptor conditional knockout mice using in vitro and in vivo approaches. J Physiol. 2014 Aug 1;592(Pt 15):3201-14
• All: 13 reference(s)