Rhot1^tm1.1Jmsu
Targeted Allele Detail

Summary

• Symbol: Rhot1^tm1.1Jmsu
• Name: ras homolog family member T1; targeted mutation 1.1, Janet M Shaw
• MGI ID: MGI:5619347
• Synonyms: Miro1^F, Miro1^loxP
• Gene: Rhot1 Location: Chr11:80099845-80158733 bp, + strand Genetic Position: Chr11, 47.62 cM, cytoband B5
• Alliance: Rhot1^tm1.1Jmsu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216408
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6 x 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site, FRT-flanked neomycin resistance gene, exon 2, and a loxP site replaced exon 2. The FRT-flanked neomycin resistance gene was removed via Flp-mediated recombination leaving exon 2 flanked by loxP sites. (J:216408)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhot1 Mutation: 43 strains or lines available

Notes

The ES cell used to generate this allele was derived from a segregating B6;129 background whereas that used to generate the tm2Jmsu allele was from a pure C57BL/6 background. The same construct was used in both targeted alleles.

References

• Original: J:216408 Nguyen TT, et al., Loss of Miro1-directed mitochondrial movement results in a novel murine model for neuron disease. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):E3631-40
• All: 2 reference(s)