Gorasp1^{tm1a(KOMP)Catr}
Targeted Allele Detail

Summary

• Symbol: Gorasp1^{tm1a(KOMP)Catr}
• Name: golgi reassembly stacking protein 1; targeted mutation 1a, Catherine Rabouille
• MGI ID: MGI:5618686
• Synonyms: Grasp65[LacZ]
• Gene: Gorasp1 Location: Chr9:119754739-119766624 bp, - strand Genetic Position: Chr9, 71.33 cM
• Alliance: Gorasp1^{tm1a(KOMP)Catr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211288
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 119757749 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 119759813. Exons 4 to 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:211288)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gorasp1 Mutation: 14 strains or lines available

References

• Original: J:211288 Veenendaal T, et al., GRASP65 controls the cis Golgi integrity in vivo. Biol Open. 2014;3(6):431-43
• All: 2 reference(s)